Author(s): Shubhangi H. Pawar, Anjalee A. Gite

Email(s): shpawar2009@gmail.com

DOI: 10.52711/2231-5691.2026.00017   

Address: Shubhangi H. Pawar, Anjalee A. Gite
Department of Pharmacology, Mahatma Gandhi Vidya Mandir's Pharmacy College, Mumbai-Agra Road, Nashik - 422003, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 2,     Year - 2026


ABSTRACT:
Background: Paclitaxel, an anticancer drug, can induce neuropathy and is used in animal models to evaluate new therapeutic approaches. It increases oxidative stress, a key factor in peripheral neuropathy. Medicinal and herbal drugs with antioxidant potential are proven as neuroprotective. W. somnifera have proven antioxidant, anti-inflammatory activity. This study aims to assess its efficacy in paclitaxel-induced neuropathy. Aim and Objectives: To carry out phytochemical investigation andto evaluate effect of W. somnifera on behavioral changes like heat hyperalgesia, cold allodynia, mechanical allodynia, locomotor activity, electrophysiological, biochemical, histopathological changes in Paclitaxel Induced Neuropathy in Wistar Rats. Material and Methods: Wistar rats (n=6 per group) were divided into nine groups. Neuropathy was induced with Paclitaxel (2mg/kg, i.p), administered on days 0, 2, 4 and 6. Treatment with W. somnifera (100, 200, 400mg/kg/p.o.) and Gabapentin (300mg/kg/p.o.) started after last paclitaxel injection for next 4 weeks. Behavioral studies, thermal hyperalgesia, cold allodynia, mechanical allodynia and locomotor activity were conducted weekly using hot plate, cold plate, Von Frey filaments and actophotometer respectively. Motor nerve conduction velocity was performed by 8 channel power lab, biochemical studies and histopathological studies were performed using sciatic nerve homogenate at the end of the study. Results: Various doses of W. somnifera significantly reversed the behavioural, electrophysiological, biochemical and histopathological changes induced by paclitaxel in dose dose-dependent manner. Conclusion: The results indicate that W. somnifera exhibit antioxidant, analgesic and neuroprotective effects. This study indicated W. somnifera is beneficial to protect paclitaxel induced neuropathy and could be used as new therapeutic approach along with current medicines in the treatment of peripheral neuropathy.


Cite this article:
Shubhangi H. Pawar, Anjalee A. Gite. Evaluation of Withania somnifera in Paclitaxel Induced Neuropathy in Wistar Rats. Asian Journal of Pharmaceutical Research. 2026; 16(2):115-2. doi: 10.52711/2231-5691.2026.00017

Cite(Electronic):
Shubhangi H. Pawar, Anjalee A. Gite. Evaluation of Withania somnifera in Paclitaxel Induced Neuropathy in Wistar Rats. Asian Journal of Pharmaceutical Research. 2026; 16(2):115-2. doi: 10.52711/2231-5691.2026.00017   Available on: https://www.asianjpr.com/AbstractView.aspx?PID=2026-16-2-2


REFERENCES:
1.    Finnerup NB., et al. Neuropathic pain: from mechanisms to treatment. Physiological Reviews. 2020 Nov 3; 101(1): 259-301. doi: org/10.1152/physrev.00045.2019.
2.    Finnerup NB., et al. Neuropathic pain: an updated grading system for research and clinical practice. Pain. 2016 Aug; 157(8): 1599-606. doi: 10.1097/j.pain.0000000000000492.
3.    Alles SR, Smith PA. Etiology and pharmacology of neuropathic pain. Pharmacological Reviews. 2018 Apr; 70(2): 315-47. doi: 10.1124/pr.117.014399.
4.    Callaghan BC., et al. Diabetic neuropathy: what does the future hold? Diabetologia. 2020 Jan 23; 63: 891-7. doi:  10.1007/s00125-020-05085-9.
5.    Mendlik MT, Uritsky TJ. Treatment of neuropathic pain. Current Treatment Options in Neurology. 2015 Dec; 17:1-5. doi:  10.1007/s11940-015-0381-2.
6.    Zajączkowska R., et al. Mechanisms of chemotherapy-induced peripheral neuropathy. International Journal of Molecular Sciences. 2019 Mar 22; 20(6): 1451. doi: 10.3390/ijms20061451. 
7.    Bernabeu E., et al. Paclitaxel: What has been done and the challenges remain ahead. International Journal of Pharmaceutics. 2017 Jun 30; 526(1-2): 474-95. doi: 10.1016/j.ijpharm.2017.05.016. 
8.    Zhao S., et al. Mechanisms of cancer cell death induction by paclitaxel: an updated review. Apoptosis. 2022 Oct; 27(9): 647-67. doi: 10.1007/s10495-022-01750-z.
9.    Zhang H., et al. Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy-induced peripheral neuropathy. The Journal of Pain. 2016 Jul; 17(7):775-86. doi: 10.1016/j.jpain.2016.02.011. 
10.    Tanabe Y., et al. Paclitaxel-induced peripheral neuropathy in patients receiving adjuvant chemotherapy for breast cancer. International Journal of Clinical Oncology. 2013 Feb; 18:132-8. doi: 10.1007/s10147-011-0352-x.
11.    Klein I, Lehmann HC. Pathomechanisms of paclitaxel-induced peripheral neuropathy. Toxics. 2021 Sep 22;9(10):229. doi: 10.3390/toxics9100229. 
12.    Caillaud M., et al. Formulated curcumin prevents paclitaxel-induced peripheral neuropathy through reduction in neuroinflammation by modulation of α7 nicotinic acetylcholine receptors. Pharmaceutics. 2022 Jun 17; 14(6): 1296. doi: 10.3390/pharmaceutics14061296.
13.    Li X., et al. Resveratrol inhibits paclitaxel-induced neuropathic pain by the activation of PI3K/Akt and SIRT1/PGC1α pathway. Journal of Pain Research. 2019 Mar 4:879-90. doi: 10.2147/JPR.S185873.
14.    Kaur S, Muthuraman A. Ameliorative effect of gallic acid in paclitaxel-induced neuropathic pain in mice. Toxicology Reports. 2019 Jun 7; 6:505-13. doi: 10.1016/j.toxrep.2019.06.001.
15.    Zhang XL., et al. Puerarin relieves paclitaxel-induced neuropathic pain: the role of Nav1. 8 β1 subunit of sensory neurons. Frontiers in Pharmacology. 2019 Jan 7; 9:1510. doi: 10.3389/fphar.2018.01510.
16.    Memariani Z., et al. Naringin and naringenin as anticancer agents and adjuvants in cancer combination therapy: Efficacy and molecular mechanisms of action, a comprehensive narrative review. Pharmacological Research. 2021 Sep; 171:105264. doi: 10.1016/j.phrs.2020.105264.
17.    Bhat JA., et al. Withania somnifera (L.) Dunal (Ashwagandha); current understanding and future prospect as a potential drug candidate. Frontiers in Pharmacology. 2022 Dec 12; 13:1029123. doi: 10.3389/fphar.2022.1029123. 
18.    Dar NJ, Ahmad M. Neurodegenerative diseases and Withania somnifera (L.): An update. Journal of Ethnopharmacology. 2020 Jun 28; 256:112769. doi: 10.1016/j.jep.2020.112769
19.    Mikulska, P., et al. Ashwagandha (Withania somnifera)—Current research on the health-promoting activities: a narrative review. Pharmaceutics. 2023 Mar 24;15(4): 1057. doi: 10.3390/pharmaceutics15041057.
20.    Bashir A., et al. An updated review on phytochemistry and molecular targets of Withania somnifera (L.) Dunal (Ashwagandha). Front. Pharmacol. 2023 Mar 29; 14:1049334. doi: 10.3389/fphar.2023.1049334.
21.    Fatani, A. J., et al. Neuroprotective effects of Gymnema sylvestre on streptozotocin-induced diabetic neuropathy in rats. Experimental and Therapeutic Medicine. 2015 May; 9(5): 1670–1678. doi: 10.3892/etm.2015.2305.
22.    Kamarudin NA, Markom M, Latip J. Effects of solvents and extraction methods on herbal plants Phyllanthus niruri, Orthosiphon stamineus and Labisia pumila. Indian Journal of Science and Technology. 2016 Jun 17; 9(21): 1-5. doi: 10.17485/ijst/2016/v9i21/95235.
23.    Kokate C. K. Practical Pharmacognosy. Vallabh Prakashan. New Delhi, India. 2010; 14:108,109. 
24.    Khandelwal K. R, Sethi V. K. Practical Pharmacognosy, techniques and experiments. Nirali Prakashan. Pune, Maharashtra. 2016; 27th edition: 25.1-25.6.
25.    Pawar S.H., et al. Attenuation of Hyperalgesia and Allodynia by some Phenolic Acids in Paclitaxel Induced Neuropathy. Journal of Advanced Scientific Research. 2021 Jan 19; 12(2); 363-368. doi: 10.1101/2021.01.17.427045.
26.    Stalin C., et al. Evaluation of Neuroprotective Effect of Ficus benghalensis against Alloxan Induced Diabetic Neuropathy in Rats. Int. J. Pharmacol. Phytochem. Ethnomed. 2016 Aug; 4:52-60. doi: 10.18052/www.scipress.com/IJPPE.4.52.
27.    Pawar S., et al. Effect of vanillic acid on nerve conduction velocity in chronic constriction injury model of neuropathy. Ind. J. Pharm. Educ. Res. 2020 Jan; 54:108-14. doi:
28.    Pawar S., et al. Evaluation of Combinational Therapy of L-Arginine with Some Phenolic Acids in Paclitaxel-induced Neuropathic Pain. Asian Journal of Biological and Life Sciences. 2023 May; 12(2): 287. doi: 10.5530/ajbls.2023.12.39.
29.    Pawar S., et al. Evaluation of some Phenolic acids in diabetic neuropathy. Indian J Pharm Educ Res. 2021 Mar; 55(1s): s176-83. doi: 10.5530/ijper.55.1s.48.
30.    O’leary JD, Warner DO. What do recent human studies tell us about the association between anaesthesia in young children and neurodevelopmental outcomes? BJA: British Journal of Anaesthesia. 2017 Sep 1; 119(3): 458-64. doi: 10.1093/bja/aex141. PMID: 28969310.
31.    Boyette-Davis JA., et al. Persistent chemoneuropathy in patients receiving the plant alkaloids paclitaxel and vincristine. Cancer Chemotherapy and Pharmacology. 2013 Mar; 71:619-26. doi: 10.1007/s00280-012-2047-z.
32.    Canta A., et al. Mitochondrial Dysfunction in Chemotherapy-Induced Peripheral Neuropathy (CIPN). Toxics. 2015 Jun 5;3(2):198-223. doi: 10.3390/toxics3020198.
33.    Sisignano M., et al. Mechanism-based treatment for chemotherapy-induced peripheral neuropathic pain. Nature Reviews Neurology. 2014 Dec 10; 10(12): 694-707. doi: 10.1038/nrneurol.2014.211.
34.    Wang XM., et al. Discovering cytokines as targets for chemotherapy-induced painful peripheral neuropathy. Cytokine. 2012 Jul; 59(1): 3-9. doi: 10.1016/j.cyto.2012.03.027.

Recomonded Articles:

Author(s): Sonali Syal, Vinay Pandit, M. S Ashawat

DOI: 10.5958/2231-5691.2020.00034.9         Access: Open Access Read More

Author(s): Jaya Preethi P, Padmini K, Srikanth J, Lohita M, Swetha K

DOI:         Access: Open Access Read More

Author(s): AK Meena, MM Rao, RP Meena, P Panda, Renu

DOI:         Access: Open Access Read More

Author(s): Vani Mamillapalli, Latha Sri Kondaveeti, Ratna Harika Chapala, Tejaswi Komal Sai. Sareddu, Santhi Pattipati, Padmalatha Khantamneni

DOI: 10.52711/2231-5691.2022.00014         Access: Open Access Read More

Author(s): Binoy Varghese Cheriyan, Sabartina scarlet, Priyadarshini, Shailesh joshi, Santhseelan, Sheik Mohamed

DOI: 10.5958/2231-5691.2019.00032.7         Access: Open Access Read More

Author(s): Ghanshyam Dhalendra, Trilochan Satapathy, Amit Roy

DOI:         Access: Open Access Read More

Author(s): Prathap Kumar Kothapalli, Jagadeesh. S. Sanganal, N.B. Shridhar

DOI:         Access: Open Access Read More

Author(s): S.K. Purohit, R. Solanki, V. Mathur, M. Mathur

DOI:         Access: Open Access Read More

Author(s): Muhammad Hamza Ashfaq, Amna Siddique, Sammia Shahid

DOI: 10.52711/2231-5691.2021.00021         Access: Open Access Read More

Author(s): Govind Navale, Dipak D. Patil, Aashutosh A. Patil, Ketan B. Patil, Narendra B. Patil

DOI: 10.5958/2231-5691.2019.00026.1         Access: Open Access Read More

Author(s): Venkateshwarlu Goli, Kanakam Vijay Bhaskar, Sravan Prasad Macharla, Jimmidi.Bhaskar, P. Suvarna Devi, T. Ramchander

DOI:         Access: Open Access Read More

Author(s): Naidu Narapusetty, O. Sivaiah, B. Balanasaraiah, M. Haranadhbabu, B. Prasad, B. Hosanna Crown, Ch. M. M. Prasada Rao

DOI: 10.5958/2231-5691.2017.00015.6         Access: Open Access Read More

Author(s): Kavya V. Reddy, Ashish V. Yachawad, Krushna K. Zambare, Sopan Landge

DOI: 10.5958/2231-5691.2020.00016.7         Access: Open Access Read More

Author(s): P. Lalitha, V. Sachithanandam, N. S. Swarnakumar, R. Sridhar

DOI: 10.5958/2231-5691.2019.00045.5         Access: Open Access Read More

Author(s): Debarshi Kar Mahapatra, Ruchi S. Shivhare, Pranesh Kumar

DOI: 10.5958/2231-5691.2018.00002.3         Access: Open Access Read More

Author(s): Farha Fatma, Anil Kumar

DOI: 10.52711/2231-5691.2021.00045         Access: Open Access Read More

Author(s): Ravi Kumar, Sachin Tyagi

DOI: 10.5958/2231-5691.2020.00046.5         Access: Open Access Read More

Author(s): Nikita D. Gidde, Indrayani D. Raut, Manojkumar M. Nitalikar, Shrinivas K. Mohite, Chandrakant S. Magdum

DOI: 10.52711/2231-5691.2021.00023         Access: Open Access Read More


Recent Articles




Tags